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+33 (0) 3 74 09 82 62
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Our products for in vitro diagnostic use

The medical device EndoMark® H1A kit for endocan

EndoMark® H1A or EMH1A is a ready-to-use sandwich-type immunoassay. It can analyze1 1 to 45 samples, either manually or on an automated platform developed by Biothelis.

This medical device is manufactured by Biothelis, according to European Directive 98/79 / CE.

The endocan measurement is reimbursed by the French Social Security (RIHN, code K305, BHN200)

Assay characteristics

Limit of quantification: 0.16 ng / mL

Range: 0.62 to 60 ng / mL

Within-run repeatability (% CV): <8%

Inter-series reproducibility (% CV): <12%

No interference observed with hemolyzed, lactescent or icteric plasma

Indications for the dosage of endocan

Event to be screened forTarget populationDosage prescriptionthreshold valuesExamples of possible pre-emptive treatments
Respiratory failureICU patientsOn admission then dailyIf < 5.5 ng / mL on admissionCorticosteroid therapy Fluid restriction Other
Postoperative pneumoniaCardiac Surgical PatientsBefore and 6 hours after the operationIf > 15 ng / mL 6 hours after the operationRapid microbiological analysis Digestive decontamination Antibioprophylaxis, other …
Nosocomial pneumoniaVentilated patientsAfter intubation then every dayIf > 9 ng / mLRapid microbiological analysis Antibioprophylaxis Other ..

Diagnostic performance of the endocan assay

Screening for respiratory failure in severe septic conditions

Threshold< 2,5 ng/mL< 5,5 ng/mL
Area under the curve0.930.93
Sensitivity0.451.00
Specificity1.000.77
Positive predictive value1.000.44
Negative predictive value0.911.00

Mikkelsen M et al, Lower serum endocan levels are associated with the development of acute lung injury after major trauma. J Crit Care. 2012

Palud A, et al, Evaluation of endothelial biomarkers as predictors of organ failures in septic shock patients. Cytokine. 2015

Ioakeimidou A et al, Increase of circulating endocan over sepsis follow-up is associated with progression into organ dysfunction. Eur J Clin Microbiol Infect Dis. 2017

Gaudet A et al, Low endocan levels are predictive of Acute Respiratory Distress Syndrome in severe sepsis and septic shock. J Crit Care. 2018 Oct; 47: 121-126

Post-operative pneumonia screening



Threshold

Pre-operative threshold

> 3 ng/mL

6h postoperative

15 ng/mL

Area under the curve (AUC)
0.700.75
Sensitivity0.880.41
Specificity0.570.95
Positive predictive value0.170.47
Negative predictive value0.980.94

Perrotti A, et al. Relevance of Endothelial Cell-Specific Molecule 1 (Endocan) Plasma Levels for Predicting Pulmonary Infection after Cardiac Surgery in Chronic Kidney Disease Patients: The Endolung Pilot Study. Cardiorenal Med. 2017 Dec;8(1):1-8. Perrotti A, et al. Is Endocan a Diagnostic Marker for Pneumonia After Cardiac Surgery? The ENDOLUNG Study. Ann Thorac Surg. 2018 Feb;105(2):535-541. NCT02542423 De Freitas Caires N et al. Endocan, sepsis, pneumonia, and acute respiratory distress syndrome. Crit Care. 2018 Oct 26;22(1):280.